Microbial volatiles organics compounds (mvocs) emitted from soils amended with organic wastes
Letizia Abis  1@  , Florence Lafouge, Raluca Ciuraru, Céline Dequc, Jean-Christophe Gueudet, Olivier Fanucci, Olivier Zurfluh, Sophie Sadet-Bourgeteau  2@  , Benjamin Loubet@
1 : INRA
INRA Grignon
2 : INRA Dijon, UMR 1347 Agroecology
Agrosup Dijon

Volatile organic compounds (VOCs) have a central role in environmental pollution. They influence the chemistry and the composition of the atmosphere. In particular, biogenic volatile organic compounds (bVOCs) contribute 90% to global VOCs emissions. Microorganisms contribute to bVOCs emissions and their emissions could be affected by different type of organic waste in soil. The aim of this study is focused on determining the effect of organic wastes on VOC emitted by microorganisms in soils.

The technique used for mVOC detection is the Proton Transfer Reaction-Time of Flight-Mass Spectrometry*(PTR-TOF-MS). PTR-TOF-MS technique provides on-line detection of VOC with two mains advantages: rapidity and the detection limit as low as 0.1 ppt by volume.

Five sample of the same soil with 4 different organic waste contributions were analyzed: (1)Municipal solid waste, (2)Green waste and sludge, (3)bio-waste, (4)farmyard manure and a control sample without organic waste. We also compared dry and humid conditions for each sample.

Results show that VOC emissions are higher in humid samples than dry ones. VOCs like acetone, Methyl Ethyl Ketone, acetaldehyde, toluene, ethanol and monoterpenes contribute between 60% and 80% of total emissions. Furthermore, other compounds emitted in smaller quantities are analyzed in order to identify a VOC profile belonging to each type of organic waste in soil.

Finally, perspectives concerning the study of the correlation between mVOC emissions profiles with microorganism diversity and activity in soil will be discussed. Also analysis of soils genetic content and manipulation of genetic diversity of soils will be performed.



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